Cloning of Alu‐containing cDNAs from human fibroblasts and identification of small Alu+ poly(A)+ RNAs in a variety of human normal and tumor cells
- 23 February 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 212 (2) , 208-212
- https://doi.org/10.1016/0014-5793(87)81346-7
Abstract
Two clones have been selected from a human fibroblast cDNA bank. By DNA sequencing the clones were shown to contain Alu elements located near the ends of the cDNA inserts. DNA of the clones was used for Northern blot hybridization analysis of a number of poly(A)‐containing RNAs from normal human tissues (brain, stomach, uterus, spleen, fibroblasts) and tumors (neurinoma, glioma, neuroblastoma, liposarcoma, adrenal cortex adenocarcinoma). All RNA samples reveal a heterodisperse distribution of Alu transcripts with discrete bands in the region of 7‐12 S RNA. The majority of these small poly(A)+ Alu+ RNAs contain Alu sequences only in one (canonical) orientation with functional signals including the split promoter for RNA polymerase III.Keywords
This publication has 17 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Transcriptional measurements of mouse repeated DNA sequencesNucleic Acids Research, 1985
- Major transcripts containing B1 and B2 repetitive sequences in cytoplasmic poly(A)+ RNA from mouse tissuesFEBS Letters, 1985
- Mouse ubiquitous B2 repeat in polysomal and cytoplasmic poly(A)+RNAs: uniderectional orientation and 3′-end localizationNucleic Acids Research, 1983
- Base sequence studies of 300 nucleotide renatured repeated human DNA clonesJournal of Molecular Biology, 1981
- Transcriptional analysis of interspersed repetitive polymerase III transcription units in human DNANucleic Acids Research, 1981
- Tissue-specific expression of mouse α-amylase genesJournal of Molecular Biology, 1980
- Ubiquitous, interspersed repeated sequences in mammalian genomes.Proceedings of the National Academy of Sciences, 1980
- A rapid alkaline extraction procedure for screening recombinant plasmid DNANucleic Acids Research, 1979
- A ubiquitous family of repeated DNA sequences in the human genomeJournal of Molecular Biology, 1979