A study of the irradiation of catalase by ionizing radiations in the presence of cysteine, cystine and glutathione

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Abstract
Catalase was irradi-ated with X-rays, [gamma]-rays and electrons from a linear accelerator and the effects on the activity, electrophoretic and chromatographic behavior and the UV spectrum of the enzyme were examined. Irradiation causes a profound change in these properties, but these changes are decreased by the presence of cysteine, cystine and glutathione during irradiation. In the estimation of the catalatic activity in the presence of these substances, allowance must be made for the depressive effect they have on the enzymic activity. Some aspects of this depression were studied.