Distribution of β2‐adrenergic receptor mRNA expression along the hamster nephron segments

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Abstract
Distribution of β2‐adrenergic receptor mRNA expression along the microdissected hamster nephron segments was examined by the reverse transcription‐polymerase chain reaction (RT‐PCR) technique. Conventional RT‐PCR using a set of primers on separate exons could not be applied for the detection of β2‐adrenergic receptor mRNA because of its intronless nature. We used the ‘rapid amplification of cDNA ends’ protocol [(1985) Proc. Natl. Acad. Sci. USA 85, 8998‐9002] as a maneuver for RT‐PCR of an intronless gene. Using this method, we successfully located hamster β2‐adrenergic receptor mRNA only in glomeruli and early proximal convoluted tubule along the nephron segments tested.