Tuftsin (Thr-Lys-Pro-Arg), a natural modulator of macrophage activity: further studies
- 1 September 1984
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 63 (2) , 137-142
- https://doi.org/10.1007/bf00285221
Abstract
Tuftsin, Thr-Lys-Pro-Arg, that activates macrophage functions, binds to specific receptors on these cells. The receptor capacity to bind tuftsin is diminished by prior treatment of the cells with dithiothreitol. Adherent mouse peritoneal macrophages bind tuftsin to a far less extent than non-adherent macrophages. Michaelis constant (Km) of tuftsin for phagocytic stimulation of macrophages is 111 ηM. The half maximal binding concentration of tuftsin by these cells is 117 ηM. These are similar values and indicate that full occupancy of the receptors by tuftsin is a necessary prerequisite for maximal phagocytosis.Keywords
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