Persistent Infection of HeLa Cells with Meningopneumonitis Virus

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Abstract
Summary: Meningopneumonitis virus has been adapted to growth in HeLa cells by incubating the cell-virus system for long periods of time. The adapted virus causes a persistent type of infection of such cultures in which cell growth and virus production are balanced. Two infected cultures have been maintained for periods of almost 1 year, during which time more than 100 harvests averaging 108 virus particles/ml have been made. Virus obtained from these cultures is more infectious for eggs and mice than virus grown in the allantoic cavity, on a particle basis.