THE INVITRO EFFECTS OF NEWCASTLE-DISEASE VIRUS ON THE METABOLIC AND ANTI-BACTERIAL FUNCTIONS OF HUMAN-NEUTROPHILS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 58  (2) , 221-227
Abstract
Live Newcastle disease virus (NDV) was used to investigate the in vitro effects of a viral infection on phagocytosis, chemiluminescence generation, superoxide production, O2 consumption, NADPH-oxidase activity and intracellular killing of bacteria by Ficoll-Hypaque separated human neutrophils (PMN). Phagocytosis of oil red O particles by NDV-treated PMN was inhibited by 50%. Chemiluminescence by PMN was inhibited 79% after zymosan stimulation and 86% after tetradeconyl phorbol acetate stimulation. Superoxide generation was inhibited by 68%. O2 consumption was inhibited in the presence of NDV by 37% after stimulation with phorbol myristate acetate while membrane-associated NADPH-enzyme activity was decreased by 19%. The percent of surviving intracellular Staphylococcus aureus was significantly elevated in NADV-treated PMN after 60 and 120 min incubation. Purified bacterial neuraminidase suppressed chemiluminescence while neuraminic acid blocked the effects of the virus. Infections with myxoviruses may suppress a number of vital neutrophil functions. The effects may be partly mediated by the interaction of viral neuraminidase with the external neutrophil membrane.