Characterization of Rainbow Trout C-Polysaccharide Binding Proteins.
- 1 January 1995
- journal article
- research article
- Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science
- Vol. 57 (3) , 419-425
- https://doi.org/10.1292/jvms.57.419
Abstract
Two proteins were isolated from rainbow trout sera by affinity chromatography using C-polysaccharide-Sepharose 4B column. One was appropriate to the trout C-reactive protein (CRP) that was reported previously (Murai et al. 1990. Dev. Comp. Immunol. 14: 49-58). The other was a newly found protein that had apparent molecular weight of 135,000 on native gradient polyacrylamide gel electrophoresis, and isoelectric points of 5.2-5.8. The N-terminal sequence (twenty amino acids) of the newly found protein was similar to CRPs from other species (e.g. 44% homology with plaice CRP). On electron microscope, the newly found protein was observed as pentagonal symmetry structure.Keywords
This publication has 0 references indexed in Scilit: