The Isolation and Characterization of the Two Macromolecular Antitumor Agents from Streptomyces
- 9 September 1962
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 26 (9) , 563-571
- https://doi.org/10.1080/00021369.1962.10858018
Abstract
Purification of two antitumor macromolecules, A216 and A280 substances, from culture filtrates of Streptomyces is achieved by chromatography using ion-exchanged celluloses. The purified macromolecules appeared homogeneous are characterized as a protein from the chemical and biological properties by paper electrophoresis, paper chromatography and ultra-centrifuge. The simple method for approximation of molecular weight of a protein from distribution coefficient on gel filtration is proposed. The molecular weights of both macromolecules given by gel filtration are near to those given by ultracentrifugal analysis.This publication has 2 references indexed in Scilit:
- The characterization of proteins by electrodialysis in starch gelsBiochimica et Biophysica Acta, 1961
- Gel filtration of proteins, peptides and amino acidsBiochimica et Biophysica Acta, 1960