Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (2) , 487-506
- https://doi.org/10.1093/nar/6.2.487
Abstract
A 1700 nucleotide DNA sequence derived from Xenopus vitellogenin mRNA has been cloned in the bacterial plasmid pBR322. The identity of the cloned sequence was verified in two ways. Firstly, the plasmid DNA was shown to hybridise to an RNA of the correct size (6,700 nucleotides). This was shown by in situ hybridisation to electrophoretically separated RNA and also by the formation of "R-loops" with purified vitellogenin mRNA. Then, using a novel procedure in which plasmid DNA covalently bound to diazotised paper is used to select complementary mRNA sequences, the cloned sequence was shown to hybridise to an mRNA which directed the synthesis of vitellogenin when translated in a reticulocyte lysate cell-free system.Keywords
This publication has 25 references indexed in Scilit:
- Quantitative analysis of specific labelled RNA's using DNA covalently linked to diazobenzyloxy-methyl-paperNucleic Acids Research, 1979
- Electron‐Microscopic Demonstration of Terminal and Internal Initiation Sites for cDNA Synthesis on Vitellogenin mRNAEuropean Journal of Biochemistry, 1978
- Kinetics of estrogen induction of Xenopus laevis vitellogenin messenger RNA as measured by hybridization to complementary DNAJournal of Biological Chemistry, 1977
- Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.Proceedings of the National Academy of Sciences, 1977
- Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemGene, 1977
- Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.Proceedings of the National Academy of Sciences, 1977
- Purification and characterization of Xenopus laevis vitellogenin messenger RNA.Journal of Biological Chemistry, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- R loop mapping of the 18S and 28S sequences in the long and short repeating units of drosophila melanogaster rDNACell, 1977
- The expression of the vitellogenin geneCell, 1976