Human Gene for -Microseminoprotein: Its Promoter Structure and Chromosomal Localization
- 1 February 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 117 (2) , 346-352
- https://doi.org/10.1093/jb/117.2.346
Abstract
The gene for human β-microseminoprotein (MSP, β-inhibin, prostatic secretory protein94 or PSP94 was isolated. The nucleotide sequence of its upstream region (2,860 bp), exon 1(35 bp), and a part of the following intron (218 bp) was determined. Transient transfection analysis on MSP-expressing (PC-3) and -nonexpressing (HepG2) cells using luciferase reporter plasmids suggested that the region from -2738 to -276 is not essential for the basal promoter activity, and that the regions from -275 to -207 and from -186 to -128 function in a cell-specific manner. The chromosome locus of the gene (MSMB) was determined through application of PCR to the DNAs of rodent-human somatic cell hybrids and also by the fluorescence in situ hybridization technique to be region qll.2 of chromo some 10.Keywords
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