Oxygen transfer in a pulse bioreactor
- 5 September 1993
- journal article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 42 (6) , 724-728
- https://doi.org/10.1002/bit.260420607
Abstract
Oxygen transfer in a novel pulse bioreactor has been evaluated. The agitator consists of a series of alternately fixed and movable parallel plates mounted so that the movable plates vibrate at 30 Hz causing a pulsating fluid motion. Pure oxygen, at pressures up to 5 atm, diffuses through silicone rubber tubing that also vibrates at 30 or 60 Hz. The main feature of this bioreactor is high oxygen transfer with low shear to prevent damage to fragile animal cell membranes. We estimate that sufficient oxygen can be supplied to support over 108 cells/mL of human diploid foreskin cells growing on microcarriers. © 1993 John Wiley & Sons, Inc.Keywords
This publication has 4 references indexed in Scilit:
- Hydrodynamic effects on cells in agitated tissue culture reactorsBioprocess and Biosystems Engineering, 1986
- Mammalian cell culture: engineering principles and scale-upTrends in Biotechnology, 1983
- Oxygen demand and supply in cell cultureApplied Microbiology and Biotechnology, 1981
- The cultivation of animal cells at controlled dissolved oxygen partial pressureBiotechnology & Bioengineering, 1968