Comparative Effects of Formalin and Salt Treatments on Hatch Rate of Koi Carp Eggs

Abstract
The efficacy of sodium chloride was compared to that of formalin for improving egg hatch rate of koi, domestic mutation of the common carp Cyprinus carpio. Twelve-hour-old fertilized eggs were held in hatching trays and given a 60-min static treatment of 0, 1,000, 2,500, or 5,000 mg sodium chloride/L, or they were treated with formalin at 250 mg/L for 60 min or 1,000 mg/L for 15 min. Mean hatch rates for salt treatments at 1,000 and 2,500 mg/L and formalin treatments at 250 mg/L were 88.7%, 81.6%, and 98.8%, respectively; these rates were significantly higher than the hatch rate for untreated eggs. The mean hatch rate for untreated eggs was 64.0%, which was not significantly different from the rate for eggs treated with formalin at 1,000 mg/L for 15 min. Treatments with salt at 5,000 mg/L resulted in a mean hatch rate of 30.3%.