Host Range, General Properties, Purification, and Electron Microscopy of Hop Latent Virus

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Abstract
Hop latent virus was latent in mosaic-sensitive hop [Humulus lupulus] seedlings and produced a systemic chlorotic flecking in cultivar Cluster seedlings. The virus produced pinpoint necrotic local lesions in the primary leaves of Phaseolus vulgaris ''Kinghorn''. Chenopodium album, C. hybridum, C. quinoa, C. urbicum and Atriplex hortensis var. cupreata also were susceptible. The virus was extracted from hop seedlings by acid precipitation, sucrose-polyethylene glycol-NaCl precipitation and sucrose density-gradient centrifugation. One visible zone appeared 26/29 mm below the meniscus. The sedimentation coefficient was 176S and the normal length of the rod-shaped particle was 610 nm. The virus reacted with antiserum to potato virus M.