Molecular and Biochemical Characterization of a Distinct Type of Fructose-1,6-Bisphosphatase from Pyrococcus furiosus
Open Access
- 15 June 2002
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (12) , 3401-3405
- https://doi.org/10.1128/jb.184.12.3401-3405.2002
Abstract
The Pyrococcus furiosus fbpA gene was cloned and expressed in Escherichia coli , and the fructose-1,6-bisphosphatase produced was subsequently purified and characterized. The dimeric enzyme showed a preference for fructose-1,6-bisphosphate, with a K m of 0.32 mM and a V max of 12.2 U/mg. The P. furiosus fructose-1,6-bisphosphatase was strongly inhibited by Li + (50% inhibitory concentration, 1 mM). Based on the presence of conserved sequence motifs and the substrate specificity of the P. furiosus fructose-1,6-bisphosphatase, we propose that this enzyme belongs to a new family, class IV fructose-1,6-bisphosphatase.Keywords
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