Subcellular localization of chicken kidney aryl acylamidase activity

15 February 1980

journal article
research article
Published by Portland Press Ltd. in Biochemical Journal

Vol. 186 (2) , 507-513
https://doi.org/10.1042/bj1860507

Abstract

The intracellular localization of aryl acylamidase (aryl-acylamide amidohydrolase, EC 3.5.1.13) in chicken kidney was investigated. By separation on density gradients of the silica sol Ludox AM, the enzyme was localized in the mitochondrial fraction. This mitochondrial fraction was shown to be substantially free of lysosomal contamination. Subfractionation of the purified mitochondria indicates that the enzyme is located on the outer membrane, can be solubilized, and may be a suitable marker enzyme for kidney mitochondria.

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