Technique to Examine Human Sperm Chromosomes Using Zona-Free Hamster Eggs
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Archives of Andrology
- Vol. 12 (1) , 79-83
- https://doi.org/10.3109/01485018409161153
Abstract
In experiments with repeated semen samples from two donors, zona-free hamster eggs were used for activation of human spermatozoa to metaphase. When mean number of spermatozoa per penetrated egg was low (1.0—3.3) after insemination in vitro, further incubation resulted in activation of spermatozoa to metaphase. High sperm/egg ratios, 4.2 and 7.5, yielded no sperm chromosomes. The preincubation and insemination intervals necessary for low sperm/egg ratios were individual.Keywords
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