COMBINATION EFFECT OF CITRININ AND OTHER CHEMICALS ON RAT-KIDNEY TUMORIGENESIS

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 67  (2) , 147-+
Abstract
Histological studies were made on the nephrotoxic effect of citrinin from Penicillium citrinum on the kidneys of rats, with or without previous treatment with the nephrotoxic chemicals, N-(3,5-dichlorophenyl)succinimide (NDPS) and N-nitrosodimethylamine (DMN). Oral administration of 0.02% or 0.05% citrinin alone caused signs of kidney injury but did not induce kidney tumors. On treatment with DMN alone, 8 of 14 rats (57.1%) developed kidney tumors; 2 (14.3%) were renal cell tumors, 8 (57.1%) were embryonal cell tumors and 1 (7.1%) was a hemangioendothelioma. Kidney tumors developed in 18 of 19 rats (94.7%) and 13 of 15 rats (86.7%) by the administration of 0.02% and 0.05% citrinin, respectively, after DMN. The tumors in these 2 groups were diagnosed histologically as renal cell tumors in 18 (94.7%) in group IV and 13 (86.7%) in group III, and as embryonal cell tumors in 14 (73.7%) in group IV and 9 (60.0%) in group III. In groups treated with citrinin after DMN the incidence of renal cell tumors was greater, and the incidence of embryonal cell tumors was slightly greater than in the group treated with DMN alone. Kidney tumors developed in 4 of 18 rats (22.2%) treated with 0.02% citrinin after NDPS, but treatment with NDPS alone did not induce kidney tumors. Treatment with citrinin changes the histological type and incidence of kidney tumors in rats induced by DMN. This study confirms that citrinin in combination with NDPS can induce kidney tumor in rats, a renal cell tumor (adenoma) histologically.