Measurement of oxygen consumption of rat bone marrow cells by a polarographic method.

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Abstract
Comparison of the respiratory rates of bone marrow was made using the Yellow Springs Instrument Co. O2 monitor, Kirk microrespirometer, and the conventional Warburg apparatus. O2 uptake values were considerably higher in the O2 monitor than those obtained by manometric methods for cell concentrations ranging from 0.5 x 107 to 14 x 107. O2 consumption of bone marrow cells measured by the O2 monitor were remarkably constant over a 30-min. incubation period. Oxidative response of bone marrow cells in the O2 monitor was significantly greater in serum than in Tyrode solution. O2 uptake increased with an increase in O2 partial pressure indicating that marrow cells have a high adaptibility for utilizing available O2.

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