One-step staining of Neisseria gonorrhoeae in urethral discharge by methyl green-pyronin.
- 1 August 1980
- journal article
- research article
- Published by BMJ in Sexually Transmitted Infections
- Vol. 56 (4) , 227-229
- https://doi.org/10.1136/sti.56.4.227
Abstract
Methyl green-pyronin (MGP) was used in a 1-step procedure to stain smears of urethral discharge from 169 men. Duplicate smears were stained by Gram''s method and discharge was cultured for N. gonorrhoeae. The orgaisms were isolated from 67 specimens and intracellular diplococci were seen in 74 smears after Gram staining and in 77 after staining by MGP. More extracellular and intracellular diplococci were seen in smears stained by MGP than by Gram''s method and the proportion of polymorphonuclear leukocytes found to contain the organisms was greater after staining with MGP. Staining with MGP is simple, rapid, inexpensive and easily automated.Keywords
This publication has 1 reference indexed in Scilit:
- Evaluation of a new technique for the demonstration of gonococci and other micro-organisms in host cells.Journal of Clinical Pathology, 1976