Evaluation of Methods for the Detection and Quantitation of Serum Fibrin-Fibrinogen Degradation Products

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Abstract
Erickson, Charlotte, String, Tim, Stewart, Douglas, and Cohen, Richard J.: Evaluation of methods for the detection and quantitation of serum fibrin- fibrinogen degradation products. Am. J. Clin. Pathol. 58: 394–399, 1972. A new specially packaged hemagglutination immunoinhibition system for assaying fibrin-fibrinogen degradation products, providing all necessary reagents in a convenient and stable form, was compared with the tanned erythrocyte hemagglutination immunoinhibition assay anci the latex-bound antifibrinogen test. Serum fibrin-fibrinogen degradation product levels were measured by all three methods in 101 serum samples accumulated from patients with disseminated intravascular coagulation. Quantitative measurements of fibrin-fibrinogen degradation products by the packaged assay and the tanned erythrocyte hemagglutination immunoinhibition method showed positive correlations (r = +0.54) for both positive and negative fibrin-fibrinogen degradation product levels (p < 0.05). The new assay is a sensitive and reliable method that is readily available for quantitation of serum fibrin-fibrinogen degradation products and suitable for use by any clinical or research laboratory. Comparison of the new assay and the tanned erythrocyte hemagglutination immunoinhibition method with the serum latex-bounci antifibrinogen test indicates that a positive latex-bound antifibrinogen test reaction accurately reflects elevated fibrin-fibrinogen degradation products but a negative latex-bound antifibrinogen test reaction frequently occurs in the presence of elevated fibrin-fibrinogen degradation products and has limited diagnostic reliability in detecting them.

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