MicroRNAs Regulate Brain Morphogenesis in Zebrafish
Top Cited Papers
- 6 May 2005
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 308 (5723) , 833-838
- https://doi.org/10.1126/science.1109020
Abstract
MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) mutants that disrupt the Dicer ribonuclease III and double-stranded RNA-binding domains. Mutant embryos do not process precursor miRNAs into mature miRNAs, but injection of preprocessed miRNAs restores gene silencing, indicating that the disrupted domains are dispensable for later steps in silencing. MZdicer mutants undergo axis formation and differentiate multiple cell types but display abnormal morphogenesis during gastrulation, brain formation, somitogenesis, and heart development. Injection of miR-430 miRNAs rescues the brain defects in MZdicer mutants, revealing essential roles for miRNAs during morphogenesis.Keywords
This publication has 39 references indexed in Scilit:
- Phylogenetic Shadowing and Computational Identification of Human microRNA GenesCell, 2005
- Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA TargetsCell, 2005
- A custom microarray platform for analysis of microRNA gene expressionNature Methods, 2004
- Mechanisms of gene silencing by double-stranded RNANature, 2004
- The functions of animal microRNAsNature, 2004
- MicroRNAsCell, 2004
- The nuclear RNase III Drosha initiates microRNA processingNature, 2003
- A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of thelet-7Small Temporal RNAScience, 2001
- Genes and Mechanisms Related to RNA Interference Regulate Expression of the Small Temporal RNAs that Control C. elegans Developmental TimingCell, 2001
- Role for a bidentate ribonuclease in the initiation step of RNA interferenceNature, 2001