Discrimination between orientation and elongation of RBC in laminar flow by means of laser diffraction

Abstract
The elongation curve of RBC as determined by rheoscopy or ektacytometry (laser diffraction) resembles a rectangular hyperbola. The experimental data obtained so far included too large errors of measurement to allow precise mathematical description. The combination of laser diffraction with image analysis has improved ektacytometry considerably, such that the error of measurement is reduced to less than 0.5%. In laminar flow RBC of healthy donors are elongated elliptically (p = 0.99: p max) and the shear stress needed for half-maximum elongation (KE).

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