Rapid Liquid Chromatographic Determination of Clopidol in Chicken Muscles

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Abstract
A simple, rapid, and sensitive liquid chromatographic method is described for the quantitative determination of trace amounts of clopidol (3,5- dichloro-2,6-dimethyl-4-pyridinol) in chicken muscles. Clopidol was extracted from tissues with acetonitrile and the extract was cleaned up on an alumina column and separated on an anion exchange column, using 0.5% acetic acid in methanol as eluant. The solvent in the eluate was evaporated and the residue was dissolved in methanol containing benzamide as internal standard. Clopidol was separated on a Zorbax ODS column (4.6 × 250 mm) by using acetonitrile–0.05M phosphate buffer, pH 7.0 (15 + 85 v/v%), and detected at 270 nm with 0.005 AUFS. Recoveries of clopidol added to chicken tissues at levels of 0.02, 0.05, and 0.1 μg/g were 94.8, 94.8, and 97.0%, respectively. This method is applicable to levels as low as 0.01 ppm clopidol in chicken muscle.

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