Characterization of a Rat C6 Glioma‐Secreted Follistatin‐Related Protein (FRP)

Abstract
A protein was isolated from rat C-6 glioma-conditioned medium and was biochemically characterized. The heparin-binding protein has a native molecular mass of 55-75000 Da, a molecular mass of 40-48000 Da under denaturing conditions, and a pI of 5.0-6.0. Based on the determined partial amino acid sequences, the full length cDNA encoding the rat and human proteins were cloned. The cDNA sequences identified the isolated rat and human protein as the homologue of a recently reported mouse osteoblast-transforming-growth-factor-beta(1)-inducible protein, encoded by the TSC-36 gene [Shibanuma, M., Mashimo, J., Mita, A., Kuroki, T. and Nose, K. (1993) Eur. J. Biochem. 217, 13-19].status: publishe