Thermodynamic and kinetic properties of short RNA helices: the oligomer sequence AnGCUn
Open Access
- 1 January 1974
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (1) , 109-128
- https://doi.org/10.1093/nar/1.1.109
Abstract
We studied the thermodynamic, kinetic and optical properties of the double helices formed by the series of self-complementary oli-gonucle otides, (Ap)n GpC(pU)n, 2 ≤ n ≤ 4, and found that the shortest helix, containing just 6 base pairs, is less stable than would be predicted from the properties of the larger molecules. It also shows a markedly smaller hyperchromism on melting than expected. These anomalous properties of a short helix indicate that one cannot always assume that base pair free energies and extinction coefficient changes are independent of helix size.Keywords
This publication has 12 references indexed in Scilit:
- The Conformational Transitions in Yeast tRNAPhe as Studied with tRNAPhe FragmentsEuropean Journal of Biochemistry, 1973
- Free energy of imperfect nucleic acid helices: II. Small Hairpin LoopsJournal of Molecular Biology, 1973
- Stability of RNA hairpin loops: A6-Cm-U6Journal of Molecular Biology, 1973
- Relaxation kinetics of dimer formation by self complementary oligonucleotidesJournal of Molecular Biology, 1971
- Self-complementary oligoribonucleotides: Effects of helix defects and guanylic acid-cytidylic acid base pairsJournal of Molecular Biology, 1971
- Self-complementary oligoribonucleotides: Adenylic acid-uridylic acid block copolymersJournal of Molecular Biology, 1971
- Estimation of Secondary Structure in Ribonucleic AcidsNature, 1971
- Thermodynamics and kinetics of G·C base pairing in the isolated extra arm of serine-specific transfer RNA from yeastBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Helix formation by d(TA) oligomers: II. Analysis of the helix-coil transitions of linear and circular oligomersJournal of Molecular Biology, 1970
- The effect of trypsin digestion on the activities of polynucleotide phosphorylaseBiochemical Journal, 1967