Basic Studies for Continuous Production of L-Aspartic Acid by Immobilized Escherichia coli Cells
Open Access
- 1 January 1974
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 27 (5) , 886-889
- https://doi.org/10.1128/aem.27.5.886-889.1974
Abstract
By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg2+ was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.Keywords
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