Sandwich Embedding of Eyeball Wall for Optimal Paraffin Sections of Retina
- 1 January 1965
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 40 (1) , 19-21
- https://doi.org/10.3109/10520296509116368
Abstract
Whole human or animal eyeballs are fixed in Heidcnhain's Susa 2-4 hr, the posterior chamber then opened by removing the anterior portion of the bulb and fixation continued for 3-6 hr. Mercurial precipitates are removed by 0.5% iodine in 80% alcohol, 2 changes of 2-4 hr each. Pieces of the bulbar wall, about 3 × 5 mm, are enclosed between two slices of formalin-fixed liver (dehydrated to 80% alcohol), bound with thread, dehydrated, and infiltrated with paraffin. The thread is then removed and the block cast.Keywords
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