CDNA microarray analysis of gene expression in fibroblasts of patients with x‐linked Emery–Dreifuss muscular dystrophy
- 30 May 2002
- journal article
- research article
- Published by Wiley in Muscle & Nerve
- Vol. 25 (6) , 898-901
- https://doi.org/10.1002/mus.10085
Abstract
To clarify the molecular nature of the pathogenesis in X-linked Emery–Dreifuss muscular dystrophy (EDMD), we monitored the expression of 2400 genes in control and EDMD fibroblasts by using complementary DNA (cDNA) microarray techniques. A total of 60 genes whose expression was altered in EDMD fibroblasts when compared with control fibroblasts were identified. Twenty-eight genes whose expression was altered with the emerin deficiency were rescued by infection with a recombinant adenovirus expressing emerin. The altered expression in five genes, including the lamin A/C gene, was confirmed by reverse transcription–polymerase chain reaction. Our preliminary results suggest a correlation between disease similarity and gene expression. We conclude that the cDNA microarray is a very efficient tool to clarify genetic and pathological features of diseases. © 2002 Wiley Periodicals, Inc. Muscle Nerve 25: 898–901, 2002Keywords
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