Abstract
Two separate lines of rainbow trout (S. gairdneri) were developed for use as standard reference lines in genetic research being conducted at the US Fish and Wildlife Service Fish Genetics Laboratory, Beulah, Wyoming. Lines (1 fall-spawning and 1 winter-spawning) were developed from the mixing of unselected strains during the 1968 and 1969 spawning seasons and were maintained since by a modified random mating scheme. Production data on egg hatchability, fry survival and growth performance for the 1972-1977 year classes show a high degree of uniformity within each year class and significant year-class differences (P < 0.05) in all traits. The use of standard reference lines, such as these, allows the removal of the effects of environmental variation from long-term studies extending over multiple spawning seasons.

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