Direct Regeneration of Shoots from Hairy Root Cultures of Centaurium erythraea Inoculated with Agrobacterium rhizogenes
- 1 December 2003
- journal article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 46 (4) , 617-619
- https://doi.org/10.1023/b:biop.0000041074.81033.3a
Abstract
Stable transformation and expression of transgenes was achieved in Centaurium erythraea Gillib. using Agrobacterium rhizogenes-mediated system. Five hairy root clones exhibited the transformed phenotype. Shoot regeneration, with green organized structures, was apparent in 4 clones, after the first subculture on Murashige and Skoog (MS) half strength medium. The integration of Ri-plasmid T-DNA was confirmed by polymerase chain reaction (PCR) analyses.Keywords
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