Endothelin-3 Stimulates Prostacyclin Production in Cultured Bovine Endothelial Cells
- 1 January 1991
- journal article
- opening lecture
- Published by Wolters Kluwer Health in Journal of Cardiovascular Pharmacology
- Vol. 17, S140-142
- https://doi.org/10.1097/00005344-199100177-00038
Abstract
Summary Among three endothelin (ET) isopeptides, ET-3 shows the most potent initial depressor response through endothelium-dependent mechanism. We have recently reported that ET-3 induces receptor-mediated phosphoinositide breakdown, which increases the intra-cellular Ca2+ concentration ([Ca2+]i) and stimulates synthesis of endothelium-derived relaxing factor (EDRF), or nitric oxide (NO), in endothelial cells (ECs). We examined whether ET-3 has any effect on synthesis of prostacyclin, a potent vasodilator prostanoid in bovine ECs. ET-3 dose-dependently (10-10 −10-8M) stimulated the formation of 6-keto-prostaglandin F1α, a stable metabolite of prostacyclin, in culture media of ECs, whose stimulatory effect was inhibited by indomethacin, a cyclooxgenase inhibitor. These data suggest that ET-3 stimulates, in addition to EDRF (NO), the synthesis and release of prostacyclin.Keywords
This publication has 0 references indexed in Scilit: