Cerebrovascular Angiotensin II Receptors in Spontaneously Hypertensive Rats

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Abstract
The objective of this study was to characterize angiotensin II (AII) receptors in cerebral capillary endothelium and to examine whether the first step in AII responsiveness, namely AII receptor binding, is aberrant in cerebral microvessels obtained from adult spontaneously hypertensive rats (SHR). The binding of [3H]angiotensin II to isolated cerebrocortical microvessels from Sprague-Dawley. Wistar-Kyoto, and SHR rats was used to characterize AII receptors on these vessels. Kinetic experiments yielded an equilibrium-derived Kd (dissociation rate constant/association rate constant) very close to that obtained from Scatchard analysis of saturation binding data. The data indicated that the two normotensive control strains exhibited comparable AII receptor affinity and binding capacity. In contrast, experiments with microvessels from adult SHR indicated a significantly higher Bmax for AII receptors relative to controls. Although experiments assessing functional endothelial alterations in the SHR to AII remain to be performed, the increase in AII receptor number suggests that an abnormality in vascular AII responsiveness may play an important role in this model of hypertension.