SERIAL QUANTITATION OF IN VITRO LYMPHOCYTE RESPONSIVENESS TO CONCANAVALIN A
- 1 September 1979
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 28 (3) , 203-206
- https://doi.org/10.1097/00007890-197909000-00010
Abstract
Eight normal donors were serially tested over 1-7 mo. for their in vitro lymphocyte responsiveness (IVLR) to 50 .mu.g of concanavalin (Con) A per ml. 3H-thymidine incorporation was monitored by routine scintillation counting and compared to actual numbers of proliferating cells (lymphoblasts) as measured by laser (flow) cytometry. Determination of total cell numbers or lymphoblast numbers was superior (P < 0.0001 and P < 0.001, respectively) to 3H-thymidine incorporation as a measure of serial stability. serial stability.Keywords
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