Isolation, characterization, and crystallization of ribulosebisphosphate carboxylase from autotrophically grown Rhodospirillum rubrum
- 1 January 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 137 (1) , 490-501
- https://doi.org/10.1128/jb.137.1.490-501.1979
Abstract
Serial culture of R. rubrum with 2% CO2 in H2 as the exclusive C source resulted in a rather large fraction of the soluble protein (> 40%) being comprised of ribulosebisphosphate carboxylase [EC 4.1.1.39] (about 6-fold higher than the highest value previously reported). Isolation of the enzyme from these cells revealed that it has physical and kinetic properties similar to those previously described for the enzyme derived from cells grown on butyrate. Notably, the small subunit (which is a constituent of the carboxylase from eukaryotes and most prokaryotes) was absent in the enzyme from autotrophically grown R. rubrum. Edman degradation of the purified enzyme revealed that the NH2 terminus is free (unlike the catalytic subunit of the carboxylase from eukaryotes) and that the NH2-terminal sequence is Met-Asp-Gln-Ser-Ser-Arg-Tyr-Val-Asn-Leu-Ala-Leu-Lys-Glu-Glu-Asp-Leu-Ile-Ala-Gly-Gly-Glx-His-Val-Leu-. Crystals of the enzyme were readily obtained by dialysis against distilled water.This publication has 77 references indexed in Scilit:
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