Heat Inactivation and Determination of Serum Gamma-Glutamyltranspeptidase as a Profile in Identification of Human Alkaline Phosphatase

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Abstract
Heat inactivation was coupled with the determination of gamma-glutamyltranspeptidase in an attempt to determine the hepatic or skeletal origin, or both, of elevated alkaline phosphatase in patients studied retrospectively as well as prospectively. The residual alkaline phosphatase activities of patients with hepatic disease were found to overlap to a certain extent those of patients with osseous disease. When a patient had both hepatic and osseous disease, the results of heat inactivation demonstrated only the predominating isoenzyme. Thus, heat inactivation cannot, by itself, provide reliable information as to the hepatic or skeletal origin, or both, of elevated alkaline phosphatase activity. However, the combination of heat inactivation and determination of the liver-specific enzyme, gamma-glutamyltranspeptidase, can reliably identify the source of elevated alkaline phosphatase activity.

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