Progression of HIV-1 infection
- 1 September 1991
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 120 (3-4) , 233-240
- https://doi.org/10.1007/bf01310478
Abstract
Summary We present data on the distribution of human immunodeficiency virus (HIV-1) proviral DNA in different subsets of peripheral blood mononuclear cells (PBMCs) over an observation period of eight months. Eleven patients with well documented HIV-1 infection were studied. The PBMCs were obtained at two intervals and purified by fluorescence-activated cell sorting (FACS) after staining with FITC-labelled monoclonal antibodies. Varying numbers of FACS-sorted CD4+ cells, CD8+ cells and peripheral monocytes were assayed for HIV-1 proviral DNA (env andgag region) by PCR. Samples from patients at CDC stages II or III had to contain 103−104 cells in order to allow detection of proviral HIV-1 DNA. At CDC stage IV, however, HIV-1 DNA was detected in as few as 100 CD4+ T-lymphocytes. In contrast, in peripheral monocytes HIV-1 DNA was not regularly found. CD8+ cells did not harbor detectable amounts of proviral DNA. During an observation period of eight months, the rate of infected CD4+ T-lymphocytes increased significantly in three patients while staying constant in the remaining eight patients. This increase of the infection rate was paralleled by clinical progression in one patient and by a decrease of the absolute number of CD4+ cells in another patient. The percentage of CD4+ cells harboring the viral genome increases in the course of the disease. These results may help to explain the decrease in CD4+ T-lymphocyte counts during HIV-1 infection.Keywords
This publication has 20 references indexed in Scilit:
- High levels of unintegrated HIV-1 DNA in brain tissue of AIDS dementia patientsNature, 1990
- Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reactionArchiv für die gesamte Virusforschung, 1989
- The Reservoir for HIV-1 in Human Peripheral Blood Is a T Cell That Maintains Expression of CD4Science, 1989
- Human Immunodeficiency Virus Type 1 Infection in Homosexual Men Who Remain Seronegative for Prolonged PeriodsNew England Journal of Medicine, 1989
- DIRECT DETECTION OF HIV RNA EXPRESSION IN SEROPOSITIVE SUBJECTSThe Lancet, 1988
- Products & MaterialsScience, 1988
- ISOLATION OF HIV-1 FROM MONOCYTES BUT NOT T LYMPHOCYTESThe Lancet, 1987
- Qualitative Analysis of Immune Function in Patients with the Acquired Immunodeficiency SyndromeNew England Journal of Medicine, 1985
- Selective Tropism of Lymphadenopathy Associated Virus (LAV) for Helper-Inducer T LymphocytesScience, 1984
- T-Lymphocyte Subpopulations in Homosexual MenNew England Journal of Medicine, 1982