STUDY OF THE NORYMBERSKI METHODS FOR DETERMINATION OF 17-KETOGENIC STEROIDS (17-HYDROXYCORTICOSTEROIDS) IN URINE
- 1 February 1958
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 18 (2) , 208-221
- https://doi.org/10.1210/jcem-18-2-208
Abstract
Modified methods for the determination of 17-ketogenic steroids by direct and indirect procedures are presented. Several factors in the Zimmermann reaction were studied experimentally; namely, time and temperature of color development, composition of reagents, specificity and method for correcting for background absorbance. The stoichiometry of the bismuthate oxidation reaction was studied and shown to effect satisfactory recoveries. Conditions for quantitative reduction of 17-ketosteroids by borohydride are presented. The precision of the methods for 17-ketosteroids and 17-ketogenic steroids was determined. From a study of a limited number of urine samples, both the 17-ketosteroids and the 17-ketogenic steroids appear to be relatively stable at room temperature without added preservative.Keywords
This publication has 1 reference indexed in Scilit:
- A NEW AND SIMPLE METHOD OF PREPARING ALCOHOLIC POTASSIUM HYDROXYDE FOR 17-KETOSTEROID DETERMINATIONSActa Endocrinologica, 1952