Two-way separation of amino acids and other ninhydrin-reacting substances by high-voltage electrophoresis followed by paper chromatography

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Abstract
A technique for 2-way separation of amino acids and other ninhydrin-reacting substances by high-voltage paper electrophoresis followed by paper chromatography is described, which gives more rapid separation than 2-way paper chromatographic methods. The method is particularly useful for the study of plasma and spinal fluid, because these fluids can be deproteinized in a few minutes with picric acid, thus eliminating the tedious process of ultrafiltration. Moreover, desalting is not necessary, as it is automatically accomplished during the electrophoresis run. A map is presented showing the positions taken by 43 ninhydrin-reacting substances when separated by this method.