Production of threonine byBrevibacterium flavum containing threonine biosynthesis genes fromEscherichia coli
- 1 October 1993
- journal article
- research article
- Published by Springer Nature in Folia Microbiologica
- Vol. 38 (5) , 355-359
- https://doi.org/10.1007/bf02898754
Abstract
Genes of the threonine operon ofEscherichia coli were used for the construction of aBrevibacterium flavum strain excreting threonine. Using the shuttle vector pCEM300 and a newly constructed shuttle vector pEC71 (7.1 kb, Kmr/Nmr), various plasmids carryingE. coli thr genes were prepared. Mutants resistant to the threonine analog 2-amino-3-hydroxyvaleric acid (AHV) were isolated after the ethyl methanesulfonate treatment ofB. flavum carrying these recombinant plasmids. A mutant ofB. flavum CCM 351 carrying the cloned genesthrA andthrB accumulated 12 g/L of threonine after 48 h of cultivation.Keywords
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