The Template Activities of Nuclear RNAs from Rat Liver, Regenerating Liver and Hepatoma AH-130 Cells

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Abstract
The ability of simulating amino acid incorporation into protein by nuclear RNA from rat liver, regenerating rat liver and hepatoma cells was investigated by a protein synthesizing system with S-30 fractions from Escherichia coli. 1. The nuclear RNA from normal rat liver had the highest template activity ; about 2.2 times more stimulation of 14C-leucine incorporation into protein than that from hepatoma cells. 2. The nuclear RNA of the liver regenerated for 16 hr after hepatectomy had about 80% of template activity of that of normal rat liver. 3. In all nuclear RNAs from these three cells, the highest template activity was found in the rapidly sedimenting 35S and 45S RNA regions, and no activity was found in low molecular RNA. 4. The distribution of RNA molecular species having the capacity for stimulating 14C-leucine incorporation in nuclear RNA extracted from normal rat liver displayed a pattern with maximum activity in the 18S region, but that from hepatoma had a broad pattern with the highest activity in the 35S region. 5. Nuclei of regenerating liver had polydisperse RNA having strong template activity in the 18S and 35S regions.

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