Reverse Phase Liquid Chromatographic Determination of Sulfathiazole Residues in Honey

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Abstract
Sulfathiazole residues were extracted from honey by homogenizing samples in acetone, filtering, and then evaporating the acetone under nitrogen at 40°C. The remaining extract was transferred to a separatory funnel with IN HC1 and ethyl ether. An aliquot of the retained acid layer was screened by using the Bratton-Marshall reaction. If the test was positive, the remaining portion was analyzed directly through a μBondapak phenyl column monitored by a UV detector at 254 nm. The mobile phase was potassium phosphate monobasic in 10% acetonitrile adjusted to pH 3.0. Time for elution was 13 min. Average recoveries were 78% at the 0.1 ppm spiking level and 68% at the 1.0 ppm level. The minimum detectable amount was 0.06 ppm based on a spiked sample extract.