STUDIES WITH MURINE LPC-1 PLASMACYTOMA USING [6-14C]ARGININE

Publisher Website
Google Scholar
Abstract
[6-14C]Arginine ([6-14C]Arg) was used as an in vivo pulse label to study BALB/c murine LPC-1 plasmacytoma synthesis and secretion of its tumor-associated M component (IgG2a, .kappa.). With this isotope, an 8- to 10-fold enhancement in the labeling of the .gamma. globulin region and 10-fold reduction in the albumin labeling were observed. Production and secretion of the M component was detected (within 30 min) after cell trasfer. Only mice which received tumor cells showed significant labeling in the .gamma. globulin region 24 h after isotope injection. The labeling behavior of the tumor M component correlated with the administered cell dose. The peak heights of radioactivity in the .gamma. region increased with increments in cell number. When the percentage radioactivity diverted into M component was plotted as a function of cell dose, a linear relationship was noted. [6-14C]Arg may be used as a tool to follow the newly synthesized tumor-associated protein, and a means of estimating tumor cell number is provided.