Automatic Method for the Determination of Folin−Ciocalteu Reducing Capacity in Food Products

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Abstract
In the present work, an automatic flow procedure based on multi-syringe flow injection analysis was developed for the assessment of Folin−Ciocalteu reagent (FCR) reducing capacity in several types of food products using gallic acid as the standard. Different strategies for mixing of sample and reagent were tested (continuous flow of FCR, merging zones, and intercalated zones approaches); lower reagent consumption and higher determination throughput were attained for the merging zones approach (100 μL of sample + 100 μL of FCR). The application of the proposed method to compounds with known antioxidant activity (both phenolic and nonphenolic) and to samples (wines, beers, teas, soft drinks, and fruit juices) provided results similar to those obtained by the conventional batch method. The detection limit was 0.6 mg L-1, and the determination frequency was about 12 h-1. Good repeatability was attained (RSD < 1.3%, n = 10). Keywords: Multi-syringe flow injection; Folin−Ciocalteu reducing capacity; phenolic compounds