Spatial Patterns of Arylsulfatase mRNA Expression in Sea Urchin Embryo
- 31 January 1990
- journal article
- research article
- Published by Wiley in Development, Growth & Differentiation
- Vol. 32 (1) , 9-13
- https://doi.org/10.1111/j.1440-169x.1990.00009.x
Abstract
Previously we showed that the arylsulfatase (Ars) activity increases markedly after the mesenchyme blastula stage, and that the increase in its activity is due to initiation of the Ars gene transcription. In order to detect cells responsible for Ars mRNA production in the sea urchin embryo, we carried out in situ hybridization with the use of 35S-labeled Ars RNA probe transcribed from Ars cDNA in a vector pT7T3-19U. The Ars gene is uniformly expressed in the cells of blastula, and the expression becomes restricted to aboral ectoderm during development by gastrula stage.This publication has 10 references indexed in Scilit:
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