Zur Kenntnis des intermediären Stoffwechsels des Histidins. Über das Verhalten von Urocaninsäure im Tierkörper. 7. Mitteilung

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Abstract
Subcut. inj. of 10 [male] guinea pigs with histidine (0.3 g./100 g. body wt.) yielded urines from which 15-32% of the histidine could be recovered. The histidine was precipitated from the urine with phosphotungstic acid, the phosphotungstate decomposed with Ba(OH)2, the histidine reprecipitated with HgCl2, relib-erated with H2S, and precipitated with picrolonic acid. The picrolonate was decomposed with HC1 and the histidine ultimately isolated as the dihydrochloride, m.p. 230[degree]. A somewhat similar procedure applied to urines obtained from 6 [male] guinea pigs injected with 0.1-0.2 g. of urocanic acid/100 g. body wt. lead ultimately to the isolation of beautiful white crystals of urocanic acid nitrate, m.p. 213-215[degree]. Of the urocanic acid injected, about 60% was recovered. Urocanicase and histidase were present in all livers studied. Urocanic acid is not a likely intermediate product in the metabolism of histidine. Initial cleavage of the imidazole ring by histidase seems more probable than a-deamination to urocanic acid and subsequent degradation of the latter.

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