Separation of wild-type lipopolysaccharides by centrifugal partition chromatography: isolation of smooth form species

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Abstract
The smooth (S)-form lipopolysaccharides (LPSs) having long O-polysaccharides were successfully isolated by centrifugal partition chromatography from the co-existing rough (R)-form species from the LPSs of wild-type strains of Salmonella enterica, serovars abortus equi and friedenau, and from Citrobacter freundii. Separation is based on different partition behaviors of the R- and S- form LPS species between aqueous and organic phases. The procedure described proved to be convenient, reproducible and readily applicable to large amounts of various LPS. It is another tool to study the relationship between chemical structure and biological activity of LPS.

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