THE POLLEN‐TUBE CHEMOTROPIC FACTOR FROM ANTIRRHINUM MAJUS: BIOASSAY, EXTRACTION, AND PARTIAL PURIFICATION

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Abstract
Mascarenhas, J. P., and L. Machlis. (U. California, Berkeley.) The pollen‐tube chemotropic factor from Antirrhinum majus: bioassay, extraction, and partial purification. Amer. Jour. Bot. 49(5): 482–489. Illus. 1962.—A new method for quantitatively detecting pollen‐tube chemotropism is described. Extracts from the gynoecial tissues of snapdragon were prepared. Fractionation by paper chromatographic methods as well as by passage through Sephadex 25 indicated that the active moiety is associated with large molecules and can be partially dissociated by treatment with methanol. The active material was not found among a large number of common metabolites and plant growth hormones. It was shown that chemotropic activity was also present in nongynoecial tissues of snapdragon as well as in one other plant. This demonstration depended on using a membrane to prevent interference by pollen‐tube growth inhibitors.
Funding Information
  • National Science Foundation (G–7031)