INSULIN SENSITIVITY OF NON-OBESE ASYMPTOMATIC DIABETICS INVIVO IN RELATION TO INSULIN RESPONSIVENESS OF THEIR ADIPOSE-TISSUE INVITRO

  • 1 January 1979
    • journal article
    • research article
    • Vol. 5  (4) , 269-273
Abstract
In 24 non-obese male subjects a 2 h glucose infusion test (12 mg/kg per min) with initial bolus injection (0.33 g/kg) was performed as a test of carbohydrate tolerance. Sixteen individuals had a normal and 8 a pathological carbohydrate tolerance (asymptomatic diabetes). All subjects received a 1 h insulin infusion (two 30-min periods of 8 or 16 mU[units]/kg MC-Actrapid). After 2 days s.c. adipose tissue was removed from the abdominal wall by needle biopsy for characterization of insulin-stimulated (1-14C) glucose incorporation into triglycerides. Under in vivo conditions insulin provoked a decrease of blood glucose concentrations by 31 .+-. 3.9% and 11.6 .+-. 2.2% as well as of plasma free fatty acids levels by 60 .+-. 4.6% and 37 .+-. 6.8% in normal persons and asymptomatic diabetics, respectively (P < 0.01). In vitro the insulin-stimulated incorporation of labeled glucose into triglycerides of adipose tissue was diminished in asymptomatic diabetics. The in vivo insulin responsiveness and the in vitro insulin sensitivity of adipose tissue are reduced in early stages of diabetes. Changes in insulin target tissues are equally important in the development of carbohydrate intolerance in nonobese subjects.

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