COVALENT C-TERMINAL FIXATION OF CYANOGEN-BROMIDE PEPTIDES IN LIQUID-PHASE-SEQUENATOR

  • 1 January 1978
    • journal article
    • research article
    • Vol. 359  (8) , 1015-1018
Abstract
The covalent fixation and hydrophilization of homoserine lactone peptides, enabling complete C-terminal sequencing in the sequenator, is described. Dimethylformamide, dimethylsulfoxide and 6 M guanidine hydrochloride in water were used as solvents, ethylenediamine, hexamethylenediamine and histamine base as amino components. The diamine peptide derivative was reacted with hydrophilic isothiocyanates and the fixed peptide was sequenced to the C-terminal amino acid. Histamine reacted particularly well and the program with 0.1 N quadrol and the hydrophobic buffers was especially suitable for this derivative. The phenylthiohydantoin derivative of homoserine was obtained in good yields.

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