A Simple Plant Nutrient Solution Purification Method for Effective Removal of Trace Metals Using Controlled Pore Glass-8-Hydroxyquinoline Chelation Column Chromatography
Open Access
- 1 September 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 76 (1) , 103-105
- https://doi.org/10.1104/pp.76.1.103
Abstract
Column chelation chromatography on controlled pore glass-8-hydroxyquinoline was demonstrated to be a very efficient method for removing trace metal contaminants from concentrated macronutrient salt solutions used to prepare nutrient media. By using 63Ni and 65Zn radio-isotopes as tracers, controlled pore glass-8-hydroxyquinoline column packings were found to retain 99.9% of the radiotracer and quantitative recovery of the radioisotopes from these columns was obtained by eluting with 1.2 n HCl. This method has several advantages over liquid-liquid extraction methods of purification which previously have been used in plant micronutrient research.This publication has 4 references indexed in Scilit:
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